Harnessing Water to Enhance Quadrupolar NMR Spectroscopy and Imaging

Abstract 17O and 14N are attractive targets for in vivo NMR spectroscopy and imaging, but low gyromagnetic ratios γ and fast spin relaxation complicate observations. This work explores indirect ways of detecting some of these sites with the help of proton‐detected double resonance techniques. As standard coherence transfer methods are of limited use for such indirect detection, alternative routes for probing the quadrupolar spectra on 1H were tested. These centered on modulating the broadening effects imparted onto protons adjacent to the low‐γ species through J couplings through either continuous wave or spin‐echo double‐resonance decoupling/recoupling sequences. As in all cases, the changes imparted by these double‐resonance strategies were small due to the fast relaxation undergone by the quadrupoles, the sensitivity of these approaches was amplified by transferring their effects onto the abundant water 1H signal. These amplifications were mediated by the spontaneous exchanges that the labile 1Hs bound to 17O or 14N undergo with the water protons. In experiments designed on the basis of double‐resonance spin echoes, these enhancements were imparted by looping the transverse encodings together with multiple longitudinal storage periods, leading to decoupling‐recoupling with exchange (D‐REX) sequences. In experiments designed on the basis of continuous on/off quadrupolar decoupling, these solvent exchanges were incorporated into chemical‐exchange saturation transfer schemes, leading to decoupling‐recoupling with saturation transfer (D‐REST) sequences. Both of these variants harnessed sizable proportions of the easily detectable water signals, in order to characterize the NMR spectra and/or to image with atomic‐site specificity the 17O and 14N species.


Introduction
NMR spectroscopy and imaging (MRI) provide powerful noninvasive routes to discern molecular signatures, both in vitro and when distributed throughout living organisms. [1,2] Although numerous isotopes can be targeted by NMR/MRI most of them have low receptivity, making their observation difficult. One particularly important scenario where low sensitivity constrains a more widespread use of NMR arises for nuclides such as 14 N, 17 O or 33 S, species characterized by nuclear spin S > 1 = 2 , that are involved in numerous metabolic in vivo transformations. A common way to enhance the signal-to-noise ratio (SNR) of lowsensitivity nuclides is by coherently transferring their spectral information to a directly bound 1 H, [3] whose high gyromagnetic ratio (γ) provides a more sensitive way to detect NMR signals. Transfers to/from S > 1 = 2 nuclei, however, tend to be much less efficient than among spin-1 = 2 counterparts, [4][5][6] as rapid T 2 relaxation [7,8] makes J-based transfers inefficient [9,10] (unless they involve quaternary amine sites with a high symmetry and a relatively slow 14 N quadrupolar relaxation). [11][12][13][14] Their quadrupolar nature also endows such nuclei with relatively fast T 1 relaxation, which can be put to good use for extensive signal averaging and thereby SNR improvements. [15][16][17][18][19] This work revisits the problem of enhancing the detection of quadrupolar nuclei bound to labile protons by exploring the possibility of imparting an amplified version of the low-γ information onto the bulk water signal for a more sensitive NMR (and MRI) detection. Two routes are probed -both of them with roots in previous literature work. One is based on the seminal spin-echo double-resonance (SEDOR) experiment, [11,12,20] whereby an otherwise slow-relaxing spin (the 1 H) that is Jcoupled to a fast relaxing quadrupolar nucleus, will present altered T 2 relaxation properties when subject to doubleresonance irradiation-even in the absence of a resolved J splitting. [21] This phenomenon, introduced in the 1960s by Meiboom, [22] was used by Ronen et al. for imaging water's 17 O in an indirect detection fashion. [23][24][25] The present study seeks an amplification of this effect by interspersing it with repeated magnetization storage periods, enabling an exchange between the labile, quadrupole-encoding 1 H, and the bulk water proton signal. A second route relies on double-resonance techniques for modulating the longitudinal magnetization properties of the labile 1 H based on the S-spin characteristics. Such changes could then be passed on to the water 1 H, in ways akin to those employed in chemical exchange saturation transfer (CEST). [26][27][28][29][30] As in the latter experiment, this results in an amplification of the spectral information of the quadrupolar nucleus, and in sufficient sensitivity to perform MRI. The principles and main features of these two approaches -which we denote decoupling-recoupling with exchange (D-REX) and decoupling-recoupling with saturation transfer (D-REST) in order to stress their relation to the frequency-labeled by exchange (FLEX) [31][32][33] and CEST methods-are introduced in the next section. Thereafter we present results where the efficiency of these methods was used for mapping spectra and images of 17 O, a 0.038 % natural abundance [34,35] isotope of interest in imaging metabolic and functional processes, [15,36,37] as well for spectrally mapping and imaging various 14 N-containing biomolecules [38][39][40] and contrast agents. [41,42] In all cases, this was achieved by observing solely the signal of bulk water.

Theoretical background
The scenario that is considered here involves a quadrupolar S nucleus ( 17 O, 14 N) that is directly bound to a proton, to which it is also J-coupled. In general, the fast T 1 relaxation of the quadrupolar nucleus will prevent the observation of resolved multiplets; still, the S-spin will impart on the 1 H an additional contribution to the transverse relaxation rate, by what is called scalar relaxation of the second kind [43] 1 where T Q is the quadrupolar T 1S relaxation time (in reality an approximation to it that is valid when T 1S � T 2S [44] ), S is the quadrupole spin number, and J is the scalar coupling. Taking the 17 O spins of water in vivo as an example, T Q has been reported to be 4.47 � 0.14 ms, [45] S = 5 = 2 and J is 91 Hz; [46] when assuming a fast exchange between the 3.8 × 10 À 4 proton population bonded to these 17 Os at natural abundance with the protons in water (H 2 16 O), this leads to an average broadening of the latter signal by~1.6 Hz. As for the 14 N, it has a typical T Q of 1 ms, [47] S = 1, and J of 62 Hz; [48] when factoring its 99.6 % abundance, this leads to a~80 Hz broadening of the bonded proton. Regardless of their effective sizes, these broadening effects can be modulated by the application of an RF decoupling field on the S-spin. [44,49] If applying such field continuously and on-resonance, the 1 H broadening will be reduced to where ω 1 is the strength of the applied S-decoupling. This means that, in principle, it is possible to modulate this effect on/off and thereby highlight the S-spin, by the presence/ absence of the latter's decoupling. Kupce and Freeman took advantage of this effect for detecting 17 O in water based on steady-state acquisitions; [50] Ronen et al. took this a step further, and adopted the scheme illustrated in Figure 1a to transform these effects into sensitive probes for imaging the presence of metabolic 17 O. [23][24][25] In fact, if the 1 H involved is labile, both the magnitude of the broadening and therefore the feasibility of highlighting it by S-spin decoupling, will also be affected by its chemical exchange with the water. The effects of solvent exchanges at an intermolecular exchange rate k sw on the aforementioned transverse decoupled broadenings have also been investigated, [22,24,25] and transform Equation (2) where τ sw = 1/k sw . It follows from this that the aforementioned effects can be exploited as long as the quadrupole's relaxation time T Q @ τ sw . Eventually however, if the solvent exchange rate gets too fast, a "self-decoupling" ends up erasing this relaxation contribution. If considering bulk water, for instance, all of the above-mentioned effects will be attenuated by an intermolecular exchange rate k sw � 555 Hz under room temperature and neutral pH. [22] A possible way to overcome the T Q -and k sw -driven attenuations of the J-driven broadening, is by extending the spin-echo times; for instance for the case of water, a TE Figure 1. Pulse sequences considered in this work to highlight quadrupolar nuclei S bound to labile 1 Hs on the water resonance. a) Spin echo (SE) scheme based on the work of Ronen et al., [23] in which S-decoupling is/is not applied during an echo time TE and the difference on water is observed. b) D-REX, a FLEX-inspired scheme in which the S-spin is/is not highlighted with either hard pulses or with S-decoupling, and the whole module is repeated N times to port the S-encoding thus imparted from the S-bound proton to the water, as driven by exchanges happening during a mixing time t mix . S-encoding modes were based on either SEDOR, that is, on applying a series of π pulses i = 1 times (as in BIRD [51] ), and i = 4 or i = 8 times using xy-4 or xy-8 phase cyclings. [52] The S-effects were also introduced by using a continuous decoupling (CW) scheme; this included an i = 0 case representing a jumpÀ return (JR) sequence [53] in which τ comprises the full time in between π/2 pulses. c) D-REST, a saturation transfer scheme in which an RF pulse is applied on protons for a selective saturation, while an S-decoupling sequence is/is not applied on the S-spin.
� 1200 ms provides the scheme of Figure 1a with an observable contrast of about 10 % at natural abundance. [23] Alternatively, overall SNR might improve if the effects of exchange are ported from the transverse 1 H plane into a longitudinal axis that labels the 1 H b z magnetization. Figures 1b and 1c shows possible ways to do this, with S-spin encodings inspired on SEDOR and CEST protocols, respectively. In all instances the S-spins are targeted by a series of on/off experiments, from which differentials are taken on a readout focusing on the water resonance. The first of these experiments, decoupling-recoupling with exchange (D-REX, Figure 1b), introduces the S-spin effects with on-resonance pulses [33] acting in unison with 1 H spin-echoing pulses. Following this S-spin encoding the magnetization of the coupled 1 H is stored along the z-axis, and enabled to impart its information onto the water resonance during a period t mix . The whole scheme is then looped N times in order to magnify its effect up to the limit imposed by the water's relaxation time T 1,w [33] which, as previously derived for FLEX, [32,33] can be expressed as where i corresponds to each individual loop of the experiment up to a total of N, f is the concentration of the exchanging solute, t prep is the total preparation time, and the transverse relaxation of the labile proton T 2,H can be calculated in the presence/absence of exchange and decoupling using Equation (3). Figures 2a and 2b compare the different performances expected from the sequences introduced in Figures 1a and 1b, for the case of natural abundance water that is self-exchanging (see caption for parameters). For these estimations two different T 2 values were taken for non-17 O-bound water, akin to those in grey matter (41 ms) and CSF (500 ms), respectively, and the signal was weighted by the decay resulting from the extended encoding. For the quadrupole-encoded spin-echo experiment, the optimum sensitivity to the 17 O is obtained for TE~T2; for the T 2 = 500 ms case, this would correspond to an~0.03 % change in the intensity of the initial water signal, which by now has decayed by~63 %. By contrast, it is possible to optimize the τ, t mix and N values of the D-REX strategy as shown in Supporting Figure S1, to achieve the larger 17 O effects shown in Figure 2b. The increase in the 17 O-induced effects arises from the experiment's reliance on longitudinal storages that help preserve the water polarization. As shown in Figure S1, the 17 O recoupling on this natural abundance sample reduces the water signal by � 9 %; when taking into account the T 2 times for water in CSF and white matter, this would be the equivalent to changes of 4 % and 0.6 % of the initial water signal intensity, respectively. These calculations also predict that the D-REX approach can magnify 17 O's presence by approximately 25-fold versus the spin-echo version for T 2 = 41 ms, and by about 13fold for T 2 = 500 ms. However, it is important to note that the repeated pulsing occurring in D-REX can also result in further depletion of the signal as a result of experimental imperfec-tions. Additional information concerning the D-REX approach for different exchange and decoupling conditions are presented in Supporting Figure S2.  Figure 1a, b for 17 O in natural-abundance water. T Q = 4.4 ms, [45] J = 91 Hz, [46] k sw = 555 Hz, [22] T 1,w 1.41 s, T 2 = 41 ms (akin to gray matter [54] ) or 500 ms (as in CSF). a) Simulation expected for the spin-echo 17 O-encoded signal. [25] b) Expectations for the 17 O-encoded D-REX sequence and Equation (4), for t mix = 10 ms, τ = 1.6 ms, and optimization as in Figure S1. In (a) and (b), the responses account for the 17 O natural abundance. c) Calculation of the D-REST effect upon 14 N-encoding a dilute, 20 mM metabolite modeled on an amino acid, [47,48] with an exchange rate of 200 Hz, T Q = 1 ms, J = 62 Hz, T 1,w = 1.41 s, T 2,H = 60 ms, 99.6 % natural abundance. The dependence of this effect on the proton saturation field for a 2 s long CW pulse was explored, and the effect was combined with the overall CEST effect.
While both of these SEDOR-based strategies show promise for enhancing the detection of quadrupolar nuclei, the short T 2 values involved require the use of short τ values in order to impart significant enhancements. Therefore, these encodings are optimally performed with hard non-selective pulses-depriving the 1 H dimension of spectral resolution. This is unimportant if focusing on 17 O and bulk water; however, in some instances it would be desirable to perform frequency-selective experiments leading to a 2D-like encoding of the 1 H and S resonances. To this effect we explored the D-REST experiment shown in Figure 1c -a CEST-related approach to enhance and map specific S-spins, bound to specific labile protons. As in D-REX, this method also aims at measuring all this information on the water resonance; this time as mediated by a CEST process targeting the labile 1 H, whose efficiency will depend on whether this 1 H is or is not S-decoupled during its CW saturation. Under suitable conditions, introducing the decoupling should narrow the 1 H line being saturated, thereby bringing a change in the amount of CEST effect being transferred to water. Care needs to be exercised here to ensure that the RF field applied on the 1 H to implement its saturation does not become involved in some kind of J-transfer to the S-spin as driven by partially fulfilling a Hartmann-Hahn match; [44] in practice, CEST saturation fields were kept below the value needed to impart such effect ( Figures S3a and S3b). In such instance, the magnitude of the effect can be estimated based on the analytical expression for the width of a peak being subject to D-REST, which will be given by: [55] n 1=2 ¼ ffi ffi ffi ffi ffi ffi ffi ffi ffi ffi ffi ffi ffi ffi ffi ffi ffi ffi ffi ffi ffi ffi ffi ffi ffi ffi w 2 Here ω 1,CEST is the (continuous) RF field applied for the ffi ffi ffi ffi ffi ffi ffi ffi ffi ffi ffi ffi k sw k ws R 1;w þk ws q , and ffi ffi ffi ffi ffi ffi ffi ffi ffi ffi ffi ffi , where R 2,H , R 1,H and R 1,w are the transverse and longitudinal relaxation times of water (w) and of the labile (H) proton, and k sw /k ws are the back and forth exchange rates between the labile (solute) and water 1 Hs. S-spin decoupling will affect the R 2,H term; these effects can be calculated from Equation (3), and used to assess the strength of the ensuing effect. Whereas applying this approach to water would result in direct saturation, and thus signal depletion, D-REST can open doors to mapping other labile solutes-for instance 14 N-bound protons such as amines, amides, etc. Figure 2c shows the performance that can be expected from this approach for a prototypical NH spin pair in an amino acid, [47,48] with a moderate k sw = 200 Hz rate, T Q = 1 ms, and J = 62 Hz. While the ensuing effect on CEST will depend on the 1 H saturating field employed, simulations predict an 14 N-imposed change of � 3 % of the total water signal under these conditions -a change that should be measurable and thus open the route for 14 N-based detections. Notice that in this case the depletion of the water signal needs not be considered, as this is CEST's main observable.

Experimental Section
The schemes introduced in the previous paragraph were explored on lysine, alanine, glycine, and urea-all purchased at natural abundance from Sigma-Aldrich. Samples were prepared in Dulbecco's phosphate buffer saline (PBS) with deuterated water (D 2 O), and their pH was adjusted with HCl as indicated in the captions.
[D 6 ]DMSO) was purchased from Merck, Fluorinert TM FC-770 from 3M ® , and 17 O-enriched water from D-Chem (Israel). A healthy female C57BL/6 mouse aged � 6 months was used in an ex vivo experiment, preapproved by the Weizmann Institute's IACUC (#35520517-2). The animal was sacrificed by cervical dislocation and its brain was removed and immediately placed in its entirety in Fluorinert TM FC-770 to prevent tissue modification. Unfortunately, not being fixed before its MRI scan, brain features were blurred in the ensuing images.
NMR data were acquired on a Varian V NMRS 300/89 (301.9 MHz for 1 H, 40.9 MHz for 17 O, 21.8 MHz for 14 N) vertical bore 7 T magnet, equipped with a two channel 5 mm probe, and a two channel 10 mm probe compatible with a triple axis gradient system. Temperature was regulated with an FTS gas heater/chiller. All the sequences used herein were programmed and used as described in each caption, and can be obtained from the authors upon request. 17 O and 14 N π/2 pulses were measured to assess the necessary RF field for decoupling, being~12 and~30 μs in the 10 mm probe, respectively. Their spectra were externally referenced in relation to 1,4-dioxane [34] and ammonium chloride, [38] respectively. Typical "off" data were collected by setting the decoupling frequency 10 kHz away from the on-resonance position, to ensure that similar power depositions onto the sample as in the "on" cases were kept. Standard 1 H acquisitions were employed to readout the different water-detected experiments, using π/2 pulses of~24 μs, a 6000 Hz spectral width, 32 768 complex points, and 5 s recycle delays. Timedomain signals were Fourier transformed, phased, and their water resonances integrated in Matlab for all of the methods here described. Imaging experiments were performed using the scanner's RARE implementation, coupled with the required encoding. All images were reconstructed using Matlab through 2D FT, and difference imaging was performed by subtracting the two differently-encoded images acquired. The custom codes used to perform this processing and other calculations described herein, are also available upon request.

D-REX 17 O NMR and MRI
As a first test of the alternatives described in the theoretical background, a series of 17 O-encoded D-REX experiments were run using 17 O-enriched water mixed with DMSO. 17 O-enrichment eased the search for optimal calibration parameters, while providing a more reliable performance estimate. The sequence was optimized along the lines of the simulations shown in Supporting Figure S1; that is, by varying the recoupling time τ, the exchange time t mix and the number of loops N; also a variety of schemes for decoupling/recoupling the 17 O were tested. A summary of these results is presented in Figure 3. Figure 3a shows the magnitude of the effects for four different encoding modes, as a function of recoupling time τ. The optimal Sirradiation scheme for these conditions was found in an xy-8 CPMG loop, [52] closely followed by an xy-4 one-probably because these are likely to handle RF inhomogeneities better.
(Further evidence for this can be seen in Supporting Figure S4, where shimmed and unshimmed versions of these experiments are compared-and again xy-based versions provide the best performances). In most instances the experimentally observed optimal τ was � 2 ms; this differs from the 1.2 ms theoretically expected to maximize the coherence transfer to a spin-5 = 2 coupled by a J = 91 Hz, [4] probably reflecting the recoupling changes introduced by the 17 O relaxation and exchange averaging effects; similar observations have been made for 1 H, 17 O 2D correlation experiments. [10] Maximizing the D-REX benefits then requires repeating this process at t mix � 1/k sw intervals, and looping it up to N . t mix � T 1,w (Figure 3b, c). The resulting effect ends up reducing the water resonance bỹ 11 % -a number that is on the order of the 17 O concentration for this sample, as expected given the relatively slow exchange rate in this DMSO-based sample (see figure caption). It should be noted however, that this 11 % is measured against a reference signal S o , that has decayed to about 70 % of the original, untouched 1 H water resonance as a result of the manipulations involved in the D-REX encoding. Figure 4 presents other aspects of these methods, by comparing the ability of an optimized D-REX acquisition to detect water's 17 O, against a spin-echo counterpart (sequence in Figure 1a). Both experiments lead to an unwanted decay of the water signal: in the latter due to transverse relaxation, and in the D-REX as a result of relaxation plus the effects of the cumulative pulse imperfections (Figures 4a, 4b). Still, in the range of the useful, plateauing 17 O-encoding ( � 600 ms for the spin-echo, � 150 loops for the D-REX), the ratio of the water signal preserved in these experiments is 45 % and 70 % respectively. While as shown in Figures 4c and 4d the D-REXbased encoding leads to a � 3.5x higher sensitivity contrast for the detection of 17 O in water than its spin-echo counterpart, this enhancement is substantially smaller ( � 30 %) than what is predicted based on the aforementioned analytical expressions. We ascribe this lack in efficiency to the cumulative effect of pulse imperfections during the repeated storages of the refocused magnetization.
The contrast provided by these experiments can be employed to map an 17 O NMR spectrum. As D-REX's encoding marks the presence of 17 O but not its resonance frequency, the latter can be reintroduced by stepping, in a point-by-point fashion, the offset frequency of the decoupling pulses encoding the S-spin. Although this is reminiscent of spin tickling double resonance CW NMR experiments, [56] each of these data points is now acquired in a single scan. In approximately 10 minutes and with~5-7 % contrasts, one can thus map both 17 O-enriched as well as natural abundance water on the 1 H 2 O signal. This contrast is presented in Figure 5; notice that the D-REX contrasts are not significantly different for the natural abundance and for the enriched samples, as a result of the higher exchange rates favoring the enhancement in the former case. Increasing the strength of the RF fields employed to encode the 17 O will reduce the effective spectral resolution (Supporting Figure S5), but may also lead to a much more rapid scanning of the spectrum being sought. This could be of use in scanning natural abundance metabolites possessing relatively broad 17 O lines.
By affecting a reasonable percent of the water 1 Hs, D-REX sensitivity may be sufficient to allow some forms of 17 O imaging. To explore this option, 17 O-encoded D-REX was coupled to a RARE imaging sequence, and their potential was assessed on the two samples shown in Figure 6. For liquid tubes, D-REX provided images with a considerably high resolution-much less blurred than what common methods would furnish on 17 O;

Chemistry-A European Journal
Research Article doi.org/10.1002/chem.202201490 sensitivity was also high, with approximately 8 % of the water signal marking the 17 O for the enriched sample. These experiments were also tested on an ex vivo brain, to demonstrate signal could be obtained from it. The water contrast provided by this experiment amounted to~1-2 % (Figure 6b)-this is in line with the analytical expectations presented in Figure 2. This suggests that despite the relaxation/exchange times arising in tissues, and despite the potential effects other macromolecules present in tissue, D-REX-based 17 O imaging can be performed. Moreover, since most applications of these method would occur in conjunction with some 17 O enrichment by inhalation or injection, SNR can likely be higher and therefore translatable into in vivo studies.
In order to explore the applicability of this approach to other quadrupolar nuclei, D-REX was assayed for the 14 N encoding of a 1.86 M glycine solution (Figure 7). The method yielded a~5 % contrast on water, which given the water's decay translates into an~2.5 % initial water signal intensity effect. In an effort to achieve some spectral resolution these D-REX experiments were performed with jump-return (JR) encod-

Chemistry-A European Journal
Research Article doi.org/10.1002/chem.202201490 ing ( Figure 1b); although this preserved the 1 H chemical shift information, it also resulted in losses given the longer encoding times required by this procedure.

D-REST 14 N NMR and MRI
D-REX manages to map and image quadrupolar species like 17 O thanks to a number of assisting factors including J-couplings and fast exchanges with the water; nevertheless, it hides a proton chemical shift information that could become informative. 14 N characterizations-aided by a high natural abundance but hurt by low in vivo concentrations, by potentially fast quadrupolar relaxation, and by a wide range of exchange rates for labile amide and amine protons-raise a challenge that may benefit from a different approach. Indeed, rapid S-spin relaxation leading to self-decoupling, and 1 H resonances broadened by both exchange with the water and by scalar relaxation, may end up requiring longer D-REX evolution times to monitor the presence of recoupling, thereby suffering from more severe SNR losses. In order to permit these processes to act over long periods of time without such penalties we also assayed the experiment introduced in Figure 1c, which measures CEST Z-spectra of labile protons in the presence and absence of S-spin irradiation. Figure 8a shows the results of such a D-REST experiment, as applied for encoding the 14 N of natural abundance urea dissolved in water. When executed with the provisions described in the theoretical backgroundthat is, weak 1 H saturation fields and strong 14 N decoupling fields-this experiment resulted in a noticeable line narrowing of urea's 1 H resonance (not shown), that then reflects on a � 15 % change in the water-detected CEST response of urea. Figure 8b shows the more complex behavior observed when the same scheme is applied on lysine, a molecule possessing two kinds exchangeable of protons bound to different kind of 14 Ns: a broader 1 H resonance around 3 ppm downfield from water arising from the NH 2 α , and a narrower 1 H about 2.6 ppm downfield from water arising from the NH 2 ɛ . Upon introducing 14 N decoupling at 21 ppm (i. e., close to the NH 2 ɛ 14 N, the only one being observed [38] ), NH 2 ɛ shows the expected narrowing, while the latter barely changes; in fact, the NH 2 α CEST response seemingly decreases, due to losing part of the overlap it exhibited with the broader NH 2 ɛ peak. Still, in unison, these changes lead to an approximately + 10 % signal change compared with the same CEST experiment performed without decoupling.
Like D-REX, D-REST can also be used to map an 14 N chemical shift spectrum by keeping fixed the CEST saturating field on the labile 1 H resonance, and stepping over the 14 N decoupling frequency. Figure 9a shows how changes in the water signal intensity will reflect such spectral acquisition for a urea solution. Given the water-based sensitivity of this D-REST spectral encoding, its realization can also be extended onto an imaging  mode-without giving up the 14 N spectral selectivity given by the decoupling offset. This is demonstrated in Figure 9b, where a phantom containing a variety of 14 N-bound labile protons can yield lysine-specific images, by suitable combination of the 1 H saturation and of the 14 N decoupling resonances. These images resulted from subtracting two identical RARE scans, with onand off-resonance 14 N decoupling; being relatively fast, the sensitivity of these spectral images could naturally be improved by signal averaging.

Discussion and Conclusions
Methods for enhancing the detection of quadrupolar nuclei bound to labile protons, aimed in particular at biological translation, were introduced and demonstrated on different samples. As coherence transfer methods are difficult to realize when fast relaxing nuclei are bound to labile protons, here the information from the quadrupolar nucleus was sought by combining two phenomena: i) altering the residual broadening imparted to the quadrupolar-bound proton through partially averaged scalar J-couplings by using differential decoupling/ recoupling procedures, and ii) amplifying these differential broadening effects by exchange-driven saturation transfer processes, which ended up affecting sizable proportions of the   Figure 8, arising from stepping the 14 N decoupling frequency in a D-REST experiment over 250 ppm in 2 ppm steps. For all steps, the urea protons were saturated with a 10 Hz field at + 1 ppm downfield from water, and a 1 H water spectrum was collected thereafter. b) D-REST experiment targeting a urea/lysine/alanine phantom, incorporating a RARE block as signal acquisition. Lysine and urea were as in Figure 8; alanine was present at 100 mM and pH 2.2. The NH 2 ɛ 1 H resonance of lysine was saturated with a field of 16 Hz on resonance at + 2.6 ppm, and performed as a difference experiment between 14 N on-resonance decoupling at 28.8 ppm and an off-resonance decoupling spectrum, slice thickness = 2 mm, in-plane resolution = 117 × 117 μm 2 , 128 phase encodes, 1 scan per phase encode, RARE factor of 8, acquisition time of 1 min 4 s. All remaining conditions were as in Figure 8. water magnetization. Ultimately, it was the latter that reported on the quadrupoles-both as spectra and as images. An important shared aspect of the proposed experiments was imparting the J-related information to amplitude modulations of the labile 1 Hs' longitudinal magnetization. In the case of 17 O, where the residual broadenings were smaller, pulsed methods incorporating short periods of transverse evolution with/without decoupling, looped together with longitudinal mixing periods introduced for facilitating exchanges with the rest of the solvent, led to a variety of so-called D-REX experiments. The enhancement of this method in comparison to direct detection can be qualitatively gauged based on the differences between the gyromagnetic ratios γ of the observed nuclei, the magnifying effects of the chemical exchange, the T 2 -driven signal decay involved in the indirect detection, and the different recovery times (T R ) expected from significantly different 1 H and quadrupolar T 1 values, leading to different averages being acquired. Factoring in all of these NMR signal dependencies and noisereducing factors leads to an expected sensitivity enhancement of ffi ffi ffi ffi ffi For the aforementioned exchange rates and relaxation times, a ratio of T R where pulsing on the quadrupole is � 100 × faster than on its 1 H counterpart, and a T 2 -driven signal decay over the course of the looping of 50 %, Equation (6) leads to a D-REX enhancement of~1000 × relative to direct detection for 17 O. The approach thus leads to a larger contrast than previously discussed 17 O-encoding procedures based solely on transverse spin echoes; [23][24][25][57][58][59] although applied here for the detection of self-exchanges within water, applications to hydroxy groups-arising for example from 17 O-glucose-can also be envisioned.
A related but different approach was employed to enhance the water detection of 14 Ns adjacent to labile protons. Here the route taken involved modulating the linewidths of the labile 1 Hs, and detecting them through CEST-based water NMR/MRI. This modulation was implemented by 14 N decoupling. When implemented on an imaging mode, this D-REST strategy required addressing specific 1 H and 14 N chemical shifts, thus yielding the pseudo-2D NMR spectral editing that we sought. This in turn opens up interesting routes for imparting specificity into CEST-based methods, which suffer from significant overlap between the many metabolites present. [60][61][62] Following a similar analysis as that leading to Equation (6), the aforementioned conditions translate into an optimal enhancement of~2000 × in relation to directly detected 14 N signal. Still, for D-REST to be effective, a number of conditions need to be met: quadrupolar relaxation needs to be moderate as otherwise the J-related effects will become too small and 14 N decoupling inconsequential, and the labile 1 H has to be under sufficiently slow exchange to prevent self-decoupling, but fast enough to provide sufficient CEST enhancement onto the water signal. It remains to be seen how often this is met in relevant in vivo scenarios.
Another complication of D-REST over D-REX is the need to apply relatively long periods of heteronuclear decoupling; although requiring RF fields of only a few 100 Hz, these may be challenging to achieve even on animal-oriented scanners without too much power deposition. Tailored decoupling sequences could facilitate this. The main advantage of this method for targeting amines and amides, in comparison to D-REX, is the fact that both 1 H and 14 N chemical shifts are targeted, thus providing further chemical specificity.